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Es represent the gag sequences sampled from Cameroon in this study, while red squares represent intragene recombinant fragments in our samples. The blue squares show the new divergent branches formed by viruses sampled in this study. Sequence C.ZM.2006.ZM1464F appears to have been mis-labelled in the LANL database, and consistently groups with subtype A1. Additional file 2: Detailed phylogenetic a
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D. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.AbstractBackground: Alveolar macrophages (AM) avidly bind and ingest unopsonized inhaled particles and bacteria through class
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Ion of immortalized MARCO and SR-AI/II-deficient murine alveolar macrophage cell linesHongwei Zhou1, Amy Imrich1 and Lester Kobzik*1,Address: 1Department of Environmental Health, Harvard School of Public Health, Boston, MA, 02115, USA and 2Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA Email: Hongwei Zhou - hzhou@hsph.harvard.edu; Amy Imrich -
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Onfim I, Camacho RJ, Vandamme AM, Lemey P: Phylodynamics of the HIV-1 CRF02_AG clade in Cameroon. Infect Genet Evol 2012, 12:453?60. 20. Zhang M, Foley B, Schultz AK, Macke JP, Bulla I, Stanke M, Morgenstern B, Korber B, Leitner T: The role of recombination in the emergence of a complex and dynamic HIV epidemic. Retrovirology 2010, 7:25. 21. Carr JK, Salminen MO, Albert J, Sanders-Buell E, Gotte D
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Cias Zembe1, Eitel Mpoudi-Ngole2, Carolyn Williamson1,4 and Wendy A Burgers1*AbstractBackground: Cameroon, in west central Africa, has an extraordinary degree of HIV diversity, presenting a major challenge for the development of an effective HIV vaccine. Given the continuing need to closely monitor the emergence of new HIV variants in the country, we analyzed HIV-1 genetic diversity in 59 plasma s
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Ormation were obtained. RNA was extracted from plasma samples, reverse transcribed and PCR amplified as described previously [12] using subtype non-specific HIV-1 primers for HIV-1 full length gag [12] and nef [13] genes, and sequenced. Sequenced fragments were assembled using ChromasPro. Full length gag and nef sequences were generated and aligned using MUSCLE with manual editing in MEGA5, togeth
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Tion and analysis, decision to publish or preparation of the manuscript.Tongo et al. Virology Journal 2013, 10:29 http://www.virologyj.com/content/10/1/Page 7 of17. Montavon C, Vergne L, Bourgeois A, Mpoudi-Ngole E, Malonga-Mouellet G, Butel C, Toure-Kane C, Delaporte E, Peeters M: Identification of a new circulating recombinant form of HIV type 1, CRF11-cpx, involving subtypes A, G, J, and CRF01-
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Orrespondence: Darrin.Martin@uct.ac.za; Wendy.Burgers@uct.ac.za 3 Computational Biology Group, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa 1 Division of Medical Virology, University of Cape Town, Cape Town, South Africain west central Africa, at 5.3 [8]. This, together with the co-circulation of divergent variants of multiple clades, h

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