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Orrespondence: Darrin.Martin@uct.ac.za; Wendy.Burgers@uct.ac.za 3 Computational Biology Group, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa 1 Division of Medical Virology, University of Cape Town, Cape Town, South Africain west central Africa, at 5.3 [8]. This, together with the co-circulation of divergent variants of multiple clades, h
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Issecting the earliest evolutionary steps in the emergence of HIV-1M. Keywords: HIV-1 diversity, West central Africa, RDP3, Maximum likelihood, PHYMLFindings The Congo basin in west central Africa is thought to be the origin of HIV, where several cross-species transmission events from chimpanzees to humans occurred [1,2]. Cameroon, located in this region, has one of the most genetically diverse HI
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Kh R, Awazi B, Hewlett I: Increased genetic diversity and intersubtype recombinants of HIV-1 in blood donors from urban Cameroon. J Acquir Immune Defic Syndr 2007, 45:361?63. 6. Ndembi N, Abraha A, Pilch H, Ichimura H, Mbanya D, Kaptue L, Salata R, Arts EJ: Molecular characterization of human immunodeficiency virus type 1 (HIV-1) and HIV-2 in Yaounde, Cameroon: evidence of major drug resistance mu
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Tion and analysis, decision to publish or preparation of the manuscript.Tongo et al. Virology Journal 2013, 10:29 http://www.virologyj.com/content/10/1/Page 7 of17. Montavon C, Vergne L, Bourgeois A, Mpoudi-Ngole E, Malonga-Mouellet G, Butel C, Toure-Kane C, Delaporte E, Peeters M: Identification of a new circulating recombinant form of HIV type 1, CRF11-cpx, involving subtypes A, G, J, and CRF01-
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Orrespondence: Darrin.Martin@uct.ac.za; Wendy.Burgers@uct.ac.za 3 Computational Biology Group, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa 1 Division of Medical Virology, University of Cape Town, Cape Town, South Africain west central Africa, at 5.3 [8]. This, together with the co-circulation of divergent variants of multiple clades, h
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Equences previously identified as belonging to these known clades by constructing maximum likelihood trees from all available gag and nef sequences for each clade, and selecting one sequence from each of the up to ten most basal lineages from the root of these clades. Anonymously-donated HIV-infected blood units were collected between December 2006 and August 2007 from Yaound?Central Hospital, Cam
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Ck squares at the end of the branches represent the nef sequences sampled from Cameroon in this study, while red squares represent intragene recombinant fragments in our samples. Abbreviations HIV: Human Immunodeficiency Virus; CRF: Circulating recombinant form; URF: Unique recombinant form; RNA: Ribonucleic acid; PCR: Polymerase Chain Reaction. Competing interests The authors declare that they ha
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Two samples for which only gag or nef was typed, these were classified as belonging to CRF11_cpx. Notably, despite subtypes B and C collectively accounting for approximately 75 infections worldwide [16], none of our sequences were classified as belonging to either of these clades. In 10/46 samples from which both nef and gag sequences were analysed, they were classified as belonging to different